Publications

Original Papers

1. Toshiaki Abe, Reiko Daigaku, Xie Yuting, Yasukazu Daigaku, Nobuhiro Nagai, Hirokazu Kaji, Aya Katsuyama, Yuki Katsukura, Yasuko Izumida, Atsuko Suzuki, Shinji Yamada, Yao-Wen Chang, Keiko Terada, Sei-ichi Ishiguro, Noriko Osumi, Hiroshi Kunikata, Toru Nakazawa, “Retinal pigment epithelium specific metabolic phenotypes are regulated by high-mobility group protein N1” Invest. Ophthalmol. Vis. Sci. 66, 70 (2025).
   DOI:10.1167/iovs.66.4.70
2. Takeshi Hori, Hirokazu Kaji, “Alternatives to animal testing for assessing transplacental transfer” Translat. Regulat. Sci. 7, 1-7 (2025).
   DOI:10.33611/trs.2024-009
3. Inês M. Gonçalves, Muhammad Afzal, Nithil Kennedy, Ana Moita, Rui Lima, Serge Ostrovidov, Takeshi Hori, Yuji Nashimoto, Hirokazu Kaji, “Placental microphysiological systems: new advances on promising platforms that mimic the microenvironment of the human placenta” Lab Chip 25, 979-995 (2025). (Inside back cover article)
   DOI:10.1039/D4LC00500G
4. Xu Han, Xiao Duan, Alireza Jenabi, Rouhollah Mehdinavaz Aghdam, Fouad Al-Hakim Khalak, Mohammed Alqahtani, Mohamed Abbas, Jeong-Hui Park, Khandmaa Dashnyam, Jung-Hwan Lee, Barkan Kagan Durukan, Hilal Turkoglu Sasmazel, Serge Ostrovidov, Hirokazu Kaji, José Luis Pedraz, Seeram Ramakrishna, Hae-Won Kim, Hong-Zhuo, Ling-Na Han, Murugan Ramalingam, “3D printing for traumatic orthopedics: a review of current trends and opportunities” J. Biomed. Nanotech. 20, 1385-1407 (2024).
   DOI:10.1166/jbn.2024.3890
5. Shun Shibata, Shun Endo, Luis A. E. Nagai, Eri H. Kobayashi, Akira Oike, Norio Kobayashi, Akane Kitamura, Takeshi Hori, Yuji Nashimoto, Ryuichiro Nakato, Hirotaka Hamada, Hirokazu Kaji, Chie Kikutake, Mikita Suyama, Masatoshi Saito, Nobuo Yaegashi, Hiroaki Okae, Takahiro Arima, “Modeling embryo–endometrial interface recapitulating human embryo implantation” Sci. Adv. 10, eadi4819 (2024).
   DOI:10.1126/sciadv.adi4819
6. Takeshi Hori, Hiroaki Okae, Shun Shibata, Norio Kobayashi, Eri H Kobayashi, Akira Oike, Takahiro Arima, Hirokazu Kaji, “Trophoblast stem cell-based organoid models of the human placental barrier” Nat. Commun. 15, 962 (2024).
   DOI:10.1038/s41467-024-45279-y
7. Violeta Carvalho, Ines M. Gonçalves, Nelson Rodrigues, Paulo Sousa, Vania Pinto, Graça Minas, Hirokazu Kaji, Su Ryon Shin, Raquel O. Rodrigues, Senhorinha F.C.F. Teixeira, Rui A. Lima “Numerical evaluation and experimental validation of fluid flow behavior within an organ-on-a-chip model” Comput. Meth. Programs Biomed. 243, 107883 (2024).
   DOI:10.1016/j.cmpb.2023.107883
8. Yuji Nashimoto, An Konno, Takuto Imaizumi, Kaori Nishikawa, Kosuke Ino, Takeshi Hori, Hirokazu Kaji, Hirofumi Shintaku, Masafumi Goto, Hitoshi Shiku, “Microfluidic vascular formation model for assessing angiogenic capacities of single-islet” Biotechnol. Bioeng. 121, 1050-1059 (2024).
   DOI:10.1002/bit.28631
9. Suihong Liu, Lijia Cheng, Yakui Liu, Haiguang Zhang, Yongteng Song, Jeong-Hui Park, Khandmaa Dashnyam, Jung-Hwan Lee, Fouad Al-Hakim Khalak, Oliver Riester, Zheng Shi, Serge Ostrovidov, Hirokazu Kaji, Hans-Peter Deigner, José Luis Pedraz, Jonathan C. Knowles, Qingxi Hu, Hae-Won Kim, Murugan Ramalingam, “3D bioprinting tissue analogues: current development and translational implications” J. Tissue Eng. 14, 1-20 (2023).
   DOI:10.1177/20417314231187113
10. Serge Ostrovidov, Murugan Ramalingam, Hojae Bae, Gorka Orive, Toshinori Fujie, Takeshi Hori, Yuji Nashimoto, Xuetao Shi, Hirokazu Kaji, “Molecularly imprinted polymer-based sensors for detection of skeletal and cardiac muscle-related analytes” Sensors 23, 5625 (2023).
    DOI:10.3390/s23125625
11. Hiroya Abe, Tomoya Ina, Hirokazu Kaji, Matsuhiko Nishizawa, “Mussel-inspired interfacial ultrathin film for cellular adhesion on wrinkled surface of hydrophobic fluid” Polymer J. 55, 1231-1236 (2023).
    DOI:10.1038/s41428-023-00799-0
12. Serge Ostrovidov, Murugan Ramalingan, Hojae Bae, Gorka Orive, Toshinori Fujie, Xuetao Shi, Hirokazu Kaji, “Bioprinting and biomaterials for dental alveolar tissue regeneration” Front. Bioeng. Biotechnol. 11, 991821 (2023).
    DOI:10.3389/fbioe.2023.991821
13. Kazuya Yamashita, Serge Ostrovidov, Bibek Raut, Takeshi Hori, Yuji Nashimoto, Nobuhiro Nagai, Toshiaki Abe, Hirokazu Kaji, “Minimally invasive sub-retinal transplantation of RPE-J cells on a biodegradable composite PCL/collagen nanosheet” Cell Transplant. 32, 1-16 (2023).
    DOI:10.1177/09636897231165117
14. Murugan Ramalingam, Abinaya Jaisankar, Lijia Cheng, Sasirekha Krishnan, Liang Lan, Anwarul Hassan, Hilal Turkoglu Sasmazel, Hirokazu Kaji, Hans‑Peter Deigner, Jose Luis Pedraz, Hae‑Won Kim, Zheng Shi, Giovanna Marrazza, “Impact of nanotechnology on conventional and artificial intelligence‑based biosensing strategies for the detection of viruses” Discov. Nano 18, 58 (2023).
    DOI:10.1186/s11671-023-03842-4
15. Takeshi Hori, Yuya Ito, Bibek Raut, Serge Ostrovidov, Yuji Nashimoto, Nobuhiro Nagai, Toshiaki Abe, Hirokazu Kaji, “A three-dimensional printed refillable drug delivery device for long term sustained drug delivery to the retina” Sens. Mater. 35, 1301-1313 (2023).
    DOI:10.18494/SAM4167
16. Yuji Nashimoto, Takeshi Hori, Serge Ostrovidov, Sayaka Katagiri, Hirokazu Kaji, “Engineering oral microenvironments using microphysiological systems” Sens. Mater. 35, 1293-1299 (2023).
    DOI:10.18494/SAM4164
17. Yoshiko Hashikawa, Yuki Kato, Hirokazu Kaji, Toshiaki Abe, Nobuhiro Nagai, “A comparative study between a transscleral sustained-release device and eyedrops on intraocular distribution of carteolol hydrochloride” Heliyon 9, e14392 (2023).
    DOI:10.1016/j.heliyon.2023.e14392
18. Serge Ostrovidov, Murugan Ramalingan, Hojae Bae, Gorka Orive, Toshinori Fujie, Xuetao Shi, Hirokazu Kaji, “Latest developments in engineered skeletal muscle tissues for drug discovery and development” Expert Opin. Drug Discov. 18, 47-63 (2023).
    DOI:10.1080/17460441.2023.2160438
19. Nobuhiro Nagai, Reiko Daigaku, Remi Motoyama, Hirokazu Kaji, Toshiaki Abe, “Release of ranibizumab using a porous poly(dimethylsiloxane) capsule suppressed laser-induced choroidal neovascularization via the transscleral route” J. Mater. Sci.-Mater. Med. 34, 5 (2023).
    DOI:10.1007/s10856-022-06705-z
20. Norio Kobayashi, Hiroaki Okae, Hitoshi Hiura, Naoto Kubota, Eri H. Kobayashi, Shun Shibata, Akira Oike, Takeshi Hori, Chie Kikutake, Hirotaka Hamada, Hirokazu Kaji, Mikita Suyama, Marie-Line Bortolin-Cavaillé, Jérôme Cavaillé, Takahiro Arima, “The microRNA cluster C19MC confers differentiation potential into trophoblast lineages upon human pluripotent stem cells” Nat. Commun. 13, 3071 (2022).
    DOI:10.1038/s41467-022-30775-w
21. Inês M. Gonçalves, Raquel O. Rodrigues, Ana S. Moita, Takeshi Hori, Hirokazu Kaji, Rui A. Lima, Graça Minas, “Recent trends of biomaterials and biosensors for organ-on-chip platforms” Bioprinting 26, e00202 (2022).
    DOI:10.1016/j.bprint.2022.e00202
22. Inês M. Gonçalves, Violeta Carvalho, Raquel O. Rodrigues, Diana Pinho, Senhorinha F.C.F. Teixeira, Ana Moita, Takeshi Hori, Hirokazu Kaji, Rui Lima, Graça Minas*, “Organ-on-a-chip platforms for drug screening and delivery in tumor cells: a systematic review” Cancers 14, 935 (2022). (Cover article)
    DOI:10.3390/cancers14040935
23. Violeta Carvalho, Inês Gonçalves, Teresa Lage, Raquel O. Rodrigues, Graça Minas, Senhorinha F.C.F. Teixeira, Ana S. Moita, Takeshi Hori, Hirokazu Kaji, Rui A. Lima, “3D printing techniques and their applications to organ-on-a-chip platforms: a systematic review” Sensors 21, 3304 (2021). (Editor’s Choice Article)
    DOI:10.3390/s21093304
24. Bibek Raut, Li-Jiun Chen, Takeshi Hori, Hirokazu Kaji, “An open-source add-on EVOM® device for real-time transepithelial/endothelial electrical resistance measurements in multiple transwell samples” Micromachines 12, 282 (2021).
    DOI:10.3390/mi12030282
25. Ayako Hoshi, Nobuhiro Nagai, Reiko Daigaku, Remi Motoyama, Saaya Saijo, Hirokazu Kaji, Toshiaki Abe, “Effect of sustained insulin-releasing device made of poly(ethylene glycol) dimethacrylates on retinal function in streptozotocin-induced diabetic rats” J. Mater. Sci.-Mater. Med. 31, 52 (2020).
    DOI:10.1007/s10856-020-06392-8
26. Hideto Kojima, Bibek Raut, Li-Jiun Chen, Nobuhiro Nagai, Toshiaki Abe, Hirokazu Kaji, “A 3D printed self-sustainable cell-encapsulation drug delivery device for periocular transplant-based treatment of retinal degenerative diseases” Micromachines 11, 436 (2020).
    DOI:10.3390/mi11040436
27. Li-Jiun Chen, Bibek Raut, Nobuhiro Nagai, Toshiaki Abe, Hirokazu Kaji, “Prototyping a versatile two-layer multi-channel microfluidic device for direct cell-vessel co-culture” Micromachines 11, 79 (2020).
    DOI:10.3390/mi11010079
28. Nobuhiro Nagai, Zhaleh Kashkouli Nezhad, Reiko Daigaku, Saaya Saijo, Yuanhui Song, Keiko Terata, Ayako Hoshi, Matsuhiko Nishizawa, Toru Nakazawa, Hirokazu Kaji, Toshiaki Abe, “Transscleral sustained ranibizumab delivery using an episcleral implantable device: suppression of laser-induced choroidal neovascularization in rats” Int. J. Pharm. 567, 118458 (2019).
    DOI:10.1016/j.ijpharm.2019.118458
29. Yuto Sato, Nobuhiro Nagai, Toshiaki Abe, Hirokazu Kaji, “A multilayered sheet-type device capable of sustained drug release and deployment control” Biomed. Microdevices 21, 60 (2019).
    DOI:10.1007/s10544-019-0411-z
30. Nobuhiro Nagai, Saaya Saijo, Yuanhui Song, Hirokazu Kaji, Toshiaki Abe, “A drug refillable device consisting of a silicone reservoir and an injectable gelatin/chitosan gel for transscleral sustained drug delivery to the retina” Eur. J. Pharm. Biopharm. 136, 184-191 (2019).
    DOI:10.1016/j.ejpb.2019.01.024
31. Hirokazu Kaji, Nobuhiro Nagai, Matsuhiko Nishizawa, Toshiaki Abe, “Drug delivery devices for retinal diseases” Adv. Drug Deliv. Rev. 128, 148-157 (2018).
    DOI:10.1016/j.addr.2017.07.002
32. Taro Kondo, Zhaleh Kashkouli Nezhad, Jin Suzuki, Nobuhiro Nagai, Matsuhiko Nishizawa, Toshiaki Abe, Hirokazu Kaji, “A self-deploying drug release device using polymeric films” J. Biomed. Mater. Res. B Appl. Biomater. 106, 780-786 (2018).
    DOI:10.1002/jbm.b.33887
33. Nobuhiro Nagai, Shinji Yamada, Junichi Kawasaki, Eri Koyanagi, Saaya Saijo, Hirokazu Kaji, Matsuhiko Nishizawa, Toru Nakazawa, Toshiaki Abe, “Pharmacokinetic and safety evaluation of a transscleral sustained unoprostone release device in monkey eyes” Invest. Ophthalmol. Vis. Sci. 59, 644-652 (2018).
    DOI:10.1167/iovs.17-22429
34. Kuniaki Nagamine, Hirotaka Sato, Hiroyuki Kai, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa, “Contractile skeletal muscle cells cultured with a conducting soft wire for effective, selective stimulation” Sci. Rep. 8, 2253 (2018).
    DOI:10.1038/s41598-018-20729-y
35. Yuanhui Song, Nobuhiro Nagai, Saaya Saijo, Hirokazu Kaji, Matsuhiko Nishizawa, Toshiaki Abe, “In situ formation of injectable chitosan-gelatin hydrogels through double crosslinking for sustained intraocular drug delivery” Mater. Sci. Eng. C 88, 1-12 (2018).
    DOI:10.1016/j.msec.2018.02.022
36. Li-Jiun Chen, Hirokazu Kaji, “Modeling angiogenesis with micro- and nanotechnology” Lab Chip 17, 4186-4219 (2017).
    DOI:10.1039/C7LC00774D
37. Shinji Yamada, Nobuhiro Nagai, Saaya Saijo, Hirokazu Kaji, Matsuhiko Nishizawa, Kozoe Imura, Masafumi Goto, Toshiaki Abe, “Controlled basic fibroblast growth factor release device made of poly(ethyleneglycol) dimethacrylates for creating a subcutaneous neovascular bed for cell transplantation” J. Biomed. Mater. Res. A 105A, 3017-3024 (2017).
    DOI:10.1002/jbm.a.36153
38. Li-Jiun Chen, Shuntaro Ito, Hiroyuki Kai, Kuniaki Nagamine, Nobuhiro Nagai, Matsuhiko Nishizawa, Toshiaki Abe, Hirokazu Kaji, “Microfluidic co-cultures of retinal pigment epithelial cells and vascular endothelial cells to investigate choroidal angiogenesis” Sci. Rep. 7, 3538 (2017).
    DOI:10.1038/s41598-017-03788-5
39. Jin Suzuki, Nobuhiro Nagai, Matsuhiko Nishizawa, Toshiaki Abe, Hirokazu Kaji, “Electrochemical manipulation of cell populations supported by biodegradable polymeric nanosheets for cell transplantation therapy” Biomater. Sci. 5, 216-222 (2017). (Back cover article)
    DOI:10.1039/c6bm00852f
40. Nobuhiro Nagai, Yasuko Izumida, Yoshimasa Yamazaki, Hirokazu Kaji, Junichi Kawasaki, Matsuhiko Nishizawa, Toshiaki Abe, “Physicochemical and biological characterization of sustained isopropyl unoprostone-release device made of poly(ethyleneglycol) dimethacrylates” J. Mater. Sci.-Mater. Med. 28, 107 (2017).
    DOI:10.1007/s10856-017-5919-2
41. Serge Ostrovidov, Samad Ahadian, Javier Ramon-Azcon, Vahid Hosseini, Toshinori Fujie, S. Prakash Parthiban, Hitoshi Shiku, Tomokazu Matsue, Hirokazu Kaji, Murugan Ramalingam, Hojae Bae, Ali Khademhosseini, “Three-dimensional co-culture of C2C12/PC12 cells improves skeletal muscle tissue formation and function” J. Tissue Eng. Regen. Med. 11, 582-595 (2017).
    DOI:10.1002/term.1956
42. Nobuhiro Nagai, Eri Koyanagi, Yasuko Izumida, Junjun Liu, Aya Katsuyama, Hirokazu Kaji, Matsuhiko Nishizawa, Noriko Osumi, Mineo Kondo, Hiroko Terasaki, Yukihiko Mashima, Toru Nakazawa, Toshiaki Abe, “Long-term protection of genetically ablated rabbit retinal degeneration by sustained transscleral unoprostone delivery” Invest. Ophthalmol. Vis. Sci. 57, 6527-6538 (2016).
    DOI:10.1167/iovs.16-20453
43. Kuniaki Nagamine, Shun Chihara, Hiroyuki Kai, Hirokazu Kaji, Matsuhiko Nishizawa, “Totally shape-conformable electrode/hydrogel composite for on-skin electrophysiological measurements” Sens. Actuator B-Chem. 237, 49-53 (2016).
    DOI:10.1016/j.snb.2016.06.076
44. Nobuhiro Nagai, Satoru Iwata, Hirokazu Kaji, Kaori Sampei, Yuki Katsukura, Hideyuki Onami, Matsuhiko Nishizawa, Toru Nakazawa, Yukihiko Mashima, Toshiaki Abe, “Protective effects of sustained unoprostone delivery against retinal degeneration in S334ter rhodopsin mutant rats” J. Biomed. Mater. Res. B Appl. Biomater. 104, 1730-1737 (2016).
    DOI:10.1002/jbm.b.33522
45. Yuina Abe, Kuniaki Nagamine, Mayu Nakabayashi, Hiroyuki Kai, Hirokazu Kaji, Takeshi Yamauchi, Kenshi Yamasaki, Matsuhiko Nishizawa, “Minimally-invasive transepidermal potentiometry with microneedle salt bridge” Biomed. Microdevices 18, 55 (2016).
    DOI:10.1007/s10544-016-0080-0
46. Zhaleh Kashkouli Nezhad, Nobuhiro Nagai, Kotaro Yamamoto, Hirokazu Kaji, Matsuhiko Nishizawa, Hideyuki Saya, Toru Nakazawa, Toshiaki Abe, “Application of clotrimazole via a novel controlled release device provides potent retinal protection” J. Mater. Sci.-Mater. Med. 26, 230 (2015).
    DOI:10.1007/s10856-015-5561-9
47. Kuniaki Nagamine, Yuina Abe, Hiroyuki Kai, Hirokazu Kaji, Matsuhiko Nishizawa, “Highly stretchable cell-cultured hydrogel sheet” RSC Advances 5, 66334-66338 (2015).
    DOI:10.1039/c5ra11059a
48. Kuniaki Nagamine, Takuya Hirata, Kohei Okamoto, Yuina Abe, Hirokazu Kaji, Matsuhiko Nishizawa, “Portable micropatterns of neuronal cells supported by thin hydrogel films” ACS Biomater. Sci. Eng. 1, 329-334 (2015).
    DOI:10.1021/acsbiomaterials.5b00020
49. Vahid Hosseini, Philip Kollmannsberger, Samad Ahadian, Serge Ostrovidov, Hirokazu Kaji, Viola Vogel, Ali Khademhosseini, “Fiber-assisted molding (FAM) of surfaces with tunable curvature to guide cell alignment and complex tissue architecture” Small 10, 4851-4857 (2014).
    DOI:10.1002/smll.201400263
50. Masato Sasaki, Bijoy Chandapillai Karikkineth, Kuniaki Nagamine, Hirokazu Kaji, Keiichi Torimitsu, Matsuhiko Nishizawa, “Highly conductive stretchable and biocompatible electrode-hydrogel hybrids for advanced tissue engineering” Adv. Healthcare Mater. 3, 1919-1927 (2014).
    DOI:10.1002/adhm.201400209
51. Nobuhiro Nagai, Hirokazu Kaji, Hideyuki Onami, Yuki Katsukura, Yumi Ishikawa, Zhaleh Kashkouli Nezhad, Kaori Sampei, Satoru Iwata, Shuntaro Ito, Matsuhiko Nishizawa, Toru Nakazawa, Noriko Osumi, Yukihiko Mashima, Toshiaki Abe, “A platform for controlled dual-drug delivery to the retina: protective effects against light-induced retinal damage in rats” Adv. Healthcare Mater. 3, 1555-1560 (2014).
    DOI:10.1002/adhm.201400114
52. Kuniaki Nagamine, Kohei Okamoto, Hirokazu Kaji, Matsuhiko Nishizawa, “Bonding of synthetic hydrogels with fibrin as the glue to engineer hydrogel-based biodevices” J. Biosci. Bioeng. 118, 94-97 (2014).
    DOI:10.1016/j.jbiosc.2013.12.024
53. Samad Ahadian, Javier Ramón-Azcón, Haixin Chang, Xiaobin Liang, Hirokazu Kaji, Hitoshi Shiku, Ken Nakajima, Murugan Ramalingam, Hongkai Wu, Tomokazu Matsue, Ali Khademhosseini, “Electrically regulated differentiation of skeletal muscle cells on ultrathin graphene-based films” RSC Advances 4, 9534-9541 (2014).
    DOI:10.1039/C3RA46218H
54. Kuniaki Nagamine, Kohei Okamoto, Shingo Otani, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa, “Hydrogel-based bioassay sheets for in vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells” Biomater. Sci. 2, 252-256 (2014).
    DOI:10.1039/C3BM60179J
55. Serge Ostrovidov, Vahid Hosseini, Samad Ahadian, Toshinori Fujie, S. Prakash Parthiban, Murugan Ramalingam, Hojae Bae, Hirokazu Kaji, Ali Khademhosseini, “Skeletal muscle tissue engineering: methods to form skeletal myotubes and their applications” Tissue Eng. B 20, 403-436 (2014).
    DOI:10.1089/ten.TEB.2013.0534
56. Toshinori Fujie, Yoshihiro Mori, Shuntaro Ito, Matsuhiko Nishizawa, Hojae Bae, Nobuhiro Nagai, Hideyuki Onami, Toshiaki Abe, Ali Khademhosseini, Hirokazu Kaji, “Micropatterned polymeric nanosheets for local delivery of an engineered epithelial monolayer” Adv. Mater. 26, 1699-1705 (2014). (Featured in Global Medical Discovery, June 27, 2014)
    DOI:10.1002/adma.201304183
57. Nobuhiro Nagai, Hirokazu Kaji, Hideyuki Onami, Yumi Ishikawa, Matsuhiko Nishizawa, Noriko Osumi, Toru Nakazawa, Toshiaki Abe, “A polymeric device for controlled transscleral multi-drug delivery to the posterior segment of the eye” Acta Biomater. 10, 680-687 (2014).
    DOI:10.1016/j.actbio.2013.11.004
58. Samad Ahadian, Serge Ostrovidov, Vahid Hosseini, Hirokazu Kaji, Murugan Ramalingam, Hojae Bae, Ali Khademhosseini, “Electrical stimulation as a biomimicry tool for regulating muscle cell behavior” Organogenesis 9, 87-92 (2013).
    DOI:10.4161/org.25121
59. Toshinori Fujie, Samad Ahadian, Hao Liu, Haixin Chang, Serge Ostrovidov, Hongkai Wu, Hojae Bae, Ken Nakajima, Hirokazu Kaji, Ali Khademhosseini, “Engineered nanomembranes for directing cellular organization towards flexible biodevices” Nano Lett. 13, 3185-3192 (2013).
    DOI:10.1021/nl401237s
60. Javier Ramón-Azcón, Samad Ahadian, Mehdi Estili, Xiaobin Liang, Serge Ostrovidov, Hirokazu Kaji, Hitoshi Shiku, Murugan Ramalingam, Ken Nakajima, Yoshio Sakka, Ali Khademhosseini, Tomokazu Matsue, “Dielectrophoretically aligned carbon nanotubes to control electrical and mechanical properties of hydrogels to fabricate contractile muscle myofibers” Adv. Mater. 25, 4028-4034 (2013).
    DOI:10.1002/adma.201301300
61. Hideyuki Onami, Nobuhiro Nagai, Hirokazu Kaji, Matsuhiko Nishizawa, Yasufumi Sato, Noriko Osumi, Toru Nakazawa, Toshiaki Abe, “Transscleral sustained vasohibin-1 delivery by a novel device suppressed experimentally-induced choroidal neovascularization” PLoS ONE 8, e58580 (2013).
    DOI:10.1371/journal.pone.0058580
62. Samad Ahadian, Javier Ramón-Azcón, Serge Ostrovidov, Gulden Camci-Unal, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue, “A contactless electrical stimulator: application to fabricate functional skeletal muscle tissue” Biomed. Microdevices 15, 109-115 (2013).
    DOI:10.1007/s10544-012-9692-1
63. Samad Ahadian, Javier Ramón-Azcón, Serge Ostrovidov, Gulden Camci-Unal, Vahid Hosseini, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue, “Interdigitated array of Pt electrodes for electrical stimulation and engineering of aligned muscle tissue” Lab Chip 12, 3491-3503 (2012).
    DOI:10.1039/c2lc40479f
64. Javier Ramón-Azcón, Samad Ahadian, Raquel Obregón, Gulden Camci-Unal, Serge Ostrovidov, Vahid Hosseini, Hirokazu Kaji, Kosuke Ino, Hitoshi Shiku, Ali Khademhosseini, Tomokazu Matsue, “Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells” Lab Chip 12, 2959-2969 (2012).
    DOI:10.1039/C2LC40213K
65. Vahid Hosseini, Samad Ahadian, Serge Ostrovidov, Gulden Camci-Unal, Song Chen, Hirokazu Kaji, Murugan Ramalingam, Ali Khademhosseini, “Engineered contractile skeletal muscle tissue on a microgrooved methacrylated gelatin substrate” Tissue Eng. A 18, 2453-2465 (2012).
    DOI:10.1089/ten.TEA.2012.0181
66. Serge Ostrovidov, Nasim Annabi, Azadeh Seidi, Murugan Ramalingam, Fariba Dehghani, Hirokazu Kaji, Ali Khademhosseini, “Controlled release of drugs from gradient hydrogels for high-throughput analysis of cell-drug interactions” Anal. Chem. 84, 1302-1309 (2012).
    DOI:10.1021/ac202256c
67. Matthew J. Hancock, Fumiki Yanagawa, Yun-Ho Jang, Jiankang He, Nezamoddin N. Kachouie, Hirokazu Kaji, Ali Khademhosseini, “Designer hydrophilic regions regulate droplet shape for controlled surface patterning and 3D microgel synthesis” Small 8, 393-403 (2012). (Inside front cover article, also featured in Materials Views by Carol Stanier, Dec. 13, 2011)
    DOI:10.1002/smll.201101745
68. Amir M. Ghaemmaghami, Matthew J. Hancock, Helen Harrington, Hirokazu Kaji, Ali Khademhosseini, “Biomimetic tissues on a chip for drug discovery” Drug Discov. Today 17, 173-181 (2012).
    DOI:10.1016/j.drudis.2011.10.029
69. Azadeh Seidi, Hirokazu Kaji, Nasim Annabi, Serge Ostrovidov, Murugan Ramalingam, Ali Khademhosseini, “A microfluidic-based neurotoxin concentration gradient for the generation of an in vitro model of Parkinson’s disease” Biomicrofluidics 5, 022214 (2011).
    DOI:10.1063/1.3580756
70. Fumiki Yanagawa, Hirokazu Kaji, Yun-Ho Jang, Hojae Bae, Du Yanan, Junji Fukuda, Hao Qi, Ali Khademhosseini, “Directed assembly of cell-laden microgels for building porous three-dimensional tissue constructs” J. Biomed. Mater. Res. A 97A, 93-102 (2011).
    DOI:10.1002/jbm.a.33034
71. Takeaki Kawashima, Nobuhiro Nagai, Hirokazu Kaji, Norihiro Kumasaka, Hideyuki Onami, Yumi Ishikawa, Noriko Osumi, Matsuhiko Nishizawa, Toshiaki Abe, “A scalable controlled-release device for transscleral drug delivery to the retina” Biomaterials 32, 1950-1956 (2011).
    DOI:10.1016/j.biomaterials.2010.11.006
72. Hirokazu Kaji, Gulden Camci-Unal, Robert Langer, Ali Khademhosseini, “Engineering systems for the generation of patterned co-cultures for controlling cell-cell interactions” Biochim. Biophys. Acta-Gen. Subj. 1810, 239-250 (2011).
    DOI:10.1016/j.bbagen.2010.07.002
73. Hao Qi, Yanan Du, Lianyong Wang, Hirokazu Kaji, Hojae Bae, Ali Khademhosseini, “Patterned differentiation of individual embryoid bodies in spatially organized 3D hybrid microgels” Adv. Mater. 22, 5276-5281 (2010). (Inside front cover article)
    DOI:10.1022/adma.201002873
74. Lamya Ghenim, Hirokazu Kaji, Yu Hoshino, Takeshi Ishibashi, Vincent Haguet, Xavier Gidrol, Matsuhiko Nishizawa, “Monitoring impedance changes associated with motility and mitosis of a single cell” Lab Chip 10, 2546-2550 (2010).
    DOI:10.1039/c004115g
75. Hirokazu Kaji, Takeshi Ishibashi, Kuniaki Nagamine, Makoto Kanzaki, Matsuhiko Nishizawa, “Electrically induced contraction of C2C12 myotubes cultured on a porous membrane-based substrate with muscle tissue-like stiffness” Biomaterials 31, 6981-6986 (2010).
    DOI:10.1016/j.biomaterials.2010.05.071
76. Hirokazu Kaji, Takeshi Yokoi, Takeaki Kawashima, Matsuhiko Nishizawa, “Directing the flow of medium in controlled cocultures of HeLa cells and human umbilical vein endothelial cells with a microfluidic device” Lab Chip 10, 2374-2379 (2010).
    DOI:10.1039/c004583g
77. Soichiro Sekine, Shinya Nakanishi, Takeo Miyake, Kuniaki Nagamine, Hirokazu Kaji, Matsuhiko Nishizawa, “Electrodes combined with an agarose stamp for addressable micropatterning” Langmuir 26, 11526-11529 (2010).
    DOI:10.1021/la100735e
78. Nobuhiro Nagai, Norihiro Kumasaka, Takeaki Kawashima, Hirokazu Kaji, Matsuhiko Nishizawa, Toshiaki Abe, “Preparation and characterization of collagen microspheres for sustained release of VEGF” J. Mater. Sci.-Mater. Med. 21, 1891-1898 (2010).
    DOI:10.1007/s10856-010-4054-0
79. Kuniaki Nagamine, Takeaki Kawashima, Takeshi Ishibashi, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa, “Micropatterning Contractile C2C12 Myotubes Embedded in a Fibrin Gel” Biotechnol. Bioeng. 105, 1161-1167 (2010).
    DOI:10.1002/bit.22636
80. Takeaki Kawashima, Takeshi Yokoi, Hirokazu Kaji, Matsuhiko Nishizawa, “Transfer of two-dimensional patterns of human umbilical vein endothelial cells into fibrin gels to facilitate vessel formation” Chem. Commun. 46, 2070-2072 (2010).
    DOI:10.1039/b924397f
81. Takeo Miyake, Masato Oike, Shuhei Yoshino, Yohei Yatagawa, Keigo Haneda, Hirokazu Kaji, Matsuhiko Nishizawa, “Biofuel cell anode: NAD+/glucose dehydrogenase-coimmobilized ketjenblack electrode” Chem. Phys. Lett. 480, 123–126 (2009).
    DOI:10.1016/j.cplett.2009.08.075
82. Rui Lima, Mónica S N Oliveira, Takuji Ishikawa, Hirokazu Kaji, Shuji Tanaka, Matsuhiko Nishizawa, Takami Yamaguchi, “Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies” Biofabrication 1, 035005 (2009).
    DOI:10.1088/1758-5082/1/3/035005
83. Soichiro Sekine, Hirokazu Kaji, Matsuhiko Nishizawa, “Spatiotemporal subcellular biopatterning using an AFM-assisted electrochemical system” Electrochem. Commun. 11, 1781-1784 (2009).
    DOI:10.1016/j.elecom.2009.07.016
84. Masahiko Hashimoto, Hirokazu Kaji, Matsuhiko Nishizawa, “Selective capture of a specific cell type from mixed leucocytes in an electrode-integrated microfluidic device” Biosens. Bioelectron. 24, 2892-2897 (2009).
    DOI:10.1016/j.bios.2009.02.025
85. Hiroki Fujiwara, Takuji Ishikawa, Rui Lima, Noriaki Matsuki, Yohsuke Imai, Hirokazu Kaji, Matsuhiko Nishizawa, Takami Yamaguchi, “Red blood cell motions in high-hematocrit blood flowing through a stenosed microchannel” J. Biomech. 42, 838-843 (2009).
    DOI:10.1016/j.jbiomech.2009.01.026
86. Hirokazu Kaji, Takeshi Yokoi, Takeaki Kawashima, Matsuhiko Nishizawa, “Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates” Lab Chip 9, 427-432 (2009).
    DOI:10.1039/b812510d
87. Takeshi Ishibashi, Yu Hoshino, Hirokazu Kaji, Makoto Kanzaki, Masaaki Sato, Matsuhiko Nishizawa, “Localized electrical stimulation to C2C12 myotubes cultured on a porous membrane-based substrate” Biomed. Microdevices 11, 413-419 (2009).
    DOI:10.1007/s10544-008-9247-7
88. Matsuhiko Nishizawa, Takahiro Kitazume, Hirokazu Kaji, “Conducting polymer-based electrodes for controlling cellular functions” Electrochemistry 76, 532-534 (2008).
    DOI:10.5796/electrochemistry.76.5327
89. Hirokazu Kaji, Masahiko Hashimoto, Soichiro Sekine, Takeaki Kawashima, Matsuhiko Nishizawa, “Patterning adherent cells within microchannels by combination of electrochemical biolithography technique and repulsive dielectrophoretic force” Electrochemistry 76, 555-558 (2008).
    DOI:10.5796/electrochemistry.76.555
90. Soichiro Sekine, Hirokazu Kaji, Matsuhiko Nishizawa, “Integration of an electrochemical-based biolithography technique into an AFM system” Anal. Bioanal. Chem. 391, 2711-2716 (2008).
    DOI:10.1007/s00216-008-1952-9
91. Makoto Togo, Akimasa Takamura, Tatsuya Asai, Hirokazu Kaji, Matsuhiko Nishizawa, “Structural studies of enzyme-based microfluidic biofuel cells” J. Power Sources 178, 53-58 (2008).
    DOI:10.1016/j.jpowsour.2007.12.052
92. Masahiko Hashimoto, Hirokazu Kaji, Maria E. Kemppinen, Matsuhiko Nishizawa, “Localized immobilization of proteins onto microstructures within a preassembled microfluidic device” Sens. Actuator B-Chem. 128, 545-551 (2008).
    DOI:10.1016/j.snb.2007.07.065
93. Matsuhiko Nishizawa, Takashi Kamiya, Hyuma Nozaki, Hirokazu Kaji, “Anisotropic growth of conducting polymer along heparin-modified surfaces” Langmuir 23, 8304-8307 (2007).
    DOI:10.1021/la700716f
94. Takeshi Ishibashi, Kimiyasu Takoh, Hirokazu Kaji, Takashi Abe, Matsuhiko Nishizawa, “A porous membrane-based culture substrate for localized in situ electroporation of adherent mammalian cells” Sens. Actuator B-Chem. 128, 5-11 (2007).
    DOI:10.1016/j.snb.2007.05.027
95. Hirokazu Kaji, Soichiro Sekine, Masahiko Hashimoto, Takeaki Kawashima, Matsuhiko Nishizawa, “Stepwise formation of patterned cell co-cultures in silicone tubing” Biotechnol. Bioeng. 98, 919-925 (2007). (Featured in Spotlight in Biotechnology and Bioengineering)
    DOI:10.1002/bit.21505
96. Makoto Togo, Akimasa Takamura, Tatsuya Asai, Hirokazu Kaji, Matsuhiko Nishizawa, “An enzyme-based microfluidic biofuel cell using vitamin K3-mediated glucose oxidation” Electrochim. Acta 52, 4669-4674 (2007).
    DOI:10.1016/j.electacta.2007.01.067
97. Matsuhiko Nishizawa, Hyuma Nozaki, Hirokazu Kaji, Takahiro Kitazume, Noriyuki Kobayashi, Takeshi Ishibashi, Takashi Abe, “Electrodeposition of anchored polypyrrole film on microelectrodes and stimulation of cultured cardiac myocytes” Biomaterials 28, 1480-1485 (2007).
    DOI:10.1016/j.biomaterials.2006.11.034
98. Hirokazu Kaji, Takeaki Kawashima, Matsuhiko Nishizawa, “Patterning cellular motility using an electrochemical technique and a geometrically confined environment” Langmuir 22, 10784-10787 (2006).
    DOI:10.1021/la0610654
99. Hirokazu Kaji, Masahiko Hashimoto, Matsuhiko Nishizawa, “On-demand immobilization of protein matrixes inside a microfluidic device” Anal. Chem. 78, 5469-5473 (2006). (Featured in Lab on a Chip, vol.6, p.1116, 2006)
    DOI:10.1021/ac060304p
100. Ali Khademhosseini, Judy Yeh, George Eng, Jeffrey Karp, Hirokazu Kaji, Jeffrey Borenstein, Omid C. Farokhzad, Robert Langer, “Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays” Lab Chip 5, 1380-1386 (2005). (Cover photo article, featured as a news article in Analytical Chemistry, p.642, Feb.1, 2006, also published in the virtual RSC journal Chemical Biology)
     DOI:10.1039/b508096g
101. Hirokazu Kaji, Kazuto Tsukidate, Masahiko Hashimoto, Tomokazu Matsue, Matsuhiko Nishizawa, “Patterning the surface cytophobicity of an albumin-physisorbed substrate by electrochemical means” Langmuir 21, 6966-6969 (2005).
     DOI:10.1021/la050660n
102. Hirokazu Kaji, Kazuto Tsukidate, Tomokazu Matsue, Matsuhiko Nishizawa, “In situ control of cellular growth and migration on substrates using microelectrodes” J. Am. Chem. Soc. 126, 15026-15027 (2004).
     DOI:10.1021/ja045702t
103. Hirokazu Kaji, Masamitsu Kanada, Daisuke Oyamatsu, Tomokazu Matsue, Matsuhiko Nishizawa, “Microelectrochemical approach to induce local cell adhesion and growth on substrates” Langmuir 20, 16-19 (2004).
     DOI:10.1021/la035537f
104. Yuki Takii, Hirokazu Kaji, Tomokazu Matsue, Matsuhiko Nishizawa, “Microstamp-based micromachining for modulation of growth of cultured neuronal cells” JSME Int. J. Ser. C-Mech. Syst. Mach. Elem. Manuf. 47, 956-961 (2004).
     DOI:10.1299/jsmec.47.956
105. Hirokazu Kaji, Matsuhiko Nishizawa, Tomokazu Matsue, “Localized chemical stimulation to micropatterned cells using multiple laminar fluid flows” Lab Chip 3, 208-211 (2003). (Also published in the virtual RSC journal Chemical Biology)
     DOI:10.1039/b304350a
106. Hirokazu Kaji, Yuki Takii, Matsuhiko Nishizawa, Tomokazu Matsue, “Pharmacological characterization of micropatterned cardiac myocytes” Biomaterials 24, 4239-4244 (2003).
     DOI:10.1016/S0142-9612(03)00275-8
107. Hirokazu Kaji, Kimiyasu Takoh, Matsuhiko Nishizawa, Tomokazu Matsue, “Intracellular Ca2+ imaging for micropatterned cardiac myocytes” Biotechnol. Bioeng. 81, 748-751 (2003).
     DOI:10.1002/bit.10521
108. Matsuhiko Nishizawa, Atsushi Takahashi, Hirokazu Kaji, Tomokazu Matsue, “Micropatterned HeLa cell culture on PEG monolayer-coated glass substrates” Chem. Lett. 31, 904-905 (2002).
     DOI:10.1246/cl.2002.904